vitrinus and Teladorsagia circumcincta based on morphological characteristics of the males. ResultsĪdult worms expelled from the patients were identified as T. Phylogenetic analysis of the nucleotide sequence data was performed using MEGA 6.0 software. PCR amplification of ITS2-rDNA region was carried out, and products were sequenced. Genomic DNA was extracted from one male worm of each species. Morphological identification of the adult worms was performed using valid nematode keys. MethodsĪfter anthelmintic treatment, all fecal materials of the patients were collected up to 48 h and male adult worms were isolated. The aim of the present study was to identify the trichostrongyloid nematodes obtained from a familial infection in Guilan province, northern Iran, using morphological and molecular criteria. Also, Ostertagia species are gastrointestinal nematodes that mainly infect cattle, sheep and goats and in rare occasion humans. Different species of the genus Trichostrongylus are the most common trichostrongyloids in humans in endemic areas. Parasitic trichostrongyloid nematodes have a worldwide distribution in ruminants and frequently have been reported from humans in Middle and Far East, particularly in rural communities with poor personal hygiene and close cohabitation with herbivorous animals.
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